Recombinant DNA Principles and Methodologies
This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!
Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as
- plasmid biology
- nucleic acid biochemistry
- restriction enzymes
- cloning strategies
- gel electrophoresis
- southern and northern blotting
- preparation of probes
- phage lambda biology
- cosmids and genome analysis
- cloned gene expression
- polymerase chain reaction
- conventional and automated DNA sequencing
- site-directed mutagenesis
- and more!
Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.