Principles of Protein X-Ray Crystallography
In the 7 years since the publication of the previous edition, protein X-ray cr- tallography has made rapid progress. This was, to a large extent, triggered by the sudden growth of interest in structural genomics. Impressive technological - vancesfacilitatedthisdevelopment.ItrequiredupdatingChapter2onSourcesand Detectors. Itisnolongeranexceptiontocollectafulldatasetinafewminutesandtosolve the structure in hours. However, one bottleneck remains and that is the growth of quality crystals. Although accelerated by robotic systems, it is still a trial-a- errorprocess.Forthebene?tofthenoviceincrystallization,achapteron“Practical Protein Crystallization” written by Dr. Jeroen R. Mesters has been added. Another addition is a section on single-wavelength anomalous diffraction. This technique has gained much popularity in recent years, especially in the hi- throughput ?eld. Also SHELXD, a dual-space direct method for substructure - termination, equally useful as SnB, has now been added to Chapter 11 on Direct Methods. We are indebted to many colleagues who in some way or another assisted us in the preparation of this edition. Among them are Thomas Barends, Zbigniew Dauter, Rolf Hilgenfeld, Ankie Terwisscha van Scheltinga, Bram Schierbeek George Sheldrick, and Simon Tulloch. Jan Drenth is especially grateful to Bauke Dijkstra for the generous hospitality in his laboratory.
Provides a comprehensive treatment of crystallographic fundamentalsExtensively illustrated throughoutFurnishes appropriate theoretical background for the understanding of protein structure determination at atomic resolutionWritten by a world-renowned crystallographerA vital tool in modern biochemistry, biophysics, molecular biology, and drug development