Natural Killer Cell Protocols
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, "Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition" seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.
Remains the most comprehensive collection of methods available for studying natural killer cells in both mice and humansExamines a wide array of molecular and cellular techniques for manipulating and assaying NK cell functions both in vitro and in vivoIncludes techniques to assay NK cell phenotype, development, signaling, and functional responses to tumor cells, virus infection, and malaria infectionContains a wide array of techniques to assay NK cells by flow cytometryProvides molecular techniques to assay important genotypes, map functionally important genetic loci in mice, and identify genetic regulatory elementsSupplies several specialized protocols to identify novel ligands for NK cell receptorsIncludes specialized techniques for studying NK cells from tonsils, lymph nodes, uterine tissues, and gut mucosaIncludes clinically relevant assays of human killer cell Ig-like receptor (KIR) genotype and surface expression phenotype by flow cytometry, as well as detailed information on KIR nomenclature and ligand specificitiesHighlights information on nomenclature, cell lines, available antibodies, and receptor ligands in its valuable appendices