Flow Cytometry in Hematopathology
During the time that has elapsed between the ?rst edition and the second edition of this book, there has been considerable improvement in the incorporation of ?ow cytometry immu- phenotyping into hematology and pathology laboratories, including institutions where previous practice had relied heavily on traditional morphology and paraf?n-based immunostaining. In addition, ?ow cytometry (FCM) immunophenotyping has also gained acceptance as a useful diagnostic tool for the identi?cation of not only acute myeloid leukemias but also other myeloid disorders, both malignant and premalignant. During the same time, advances have been made in terms of instrumentation and commercially available reagents. For instance, the introduction of the T-cell receptor (TCR)-V? eight-tube kit has greatly facilitated the eval- tion of some dif?cult to diagnose mature T-cell disorders, and the implementation of the DNA dye DRAQ5 has improved the grading of lymphoma subpopulations present in heterogeneous samples. The role of FCM analysis has also progressed beyond that of establishing a diagnosis to that of monitoring disease and providing prognostic information. The second edition of this book re?ects the recent advances in the FCM analysis of he- topoietic disorders. To this end, the chapters have been revised to incorporate additional text and ?gures. The focus of the book and its organization remain unchanged, however.
Step-by-step methods for flow cytometry data analysis and interpretationEmphasis on visual pattern analysisCoverage of acute leukemias, lymphoproliferative disorders and myelodysplastic syndromesExtensive FCM graphics, including minimal residual disease detection in ALL, TCR-Vb repertoire in mature T-cell disorders and DNA content analysis in ALL and malignant lymphomas