MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
Presents fundamentals of plant miRNA biology, including pathways and approaches used for their discovery and investigation, as written by leading researchers in the fieldCovers a broad range of molecular approaches to the study of small RNA, from basic to advanced experimental and computational techniquesDetails the methods by which the expression of miRNAs and their impact on mRNA levels is characterized, including deep sequencing methods for miRNAs and target RNAsEnables a molecular biologist to design and perform experiments on the biology of plant miRNAs and small RNAs, including detection, quantification and localization, as well as approaches to data analysisIncludes applications and advanced adaptations of techniques relevant to all plant biologists