Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo
As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
A concise primer to a variety of both light and electron microscopy techniques allows the reader to make an informed decision regarding which one will be best for their own researchAn expansive introduction to the analysis of electron microscopy images prepares the reader for a variety of structure determination proceduresEach chapter starts with an overview of the material coveredAttractive layout with hyperlinks to related sections, figures and references creates a format ideal for quick and easy referencingFull methods section explains how to perform each technique coveredComprehensive explanations and over 80 illustrations lead the reader through the results presented