Antibody Expression and Production
Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.
Detailed background for antibody generation and production Chapters on production of antibody in bacterial, fungal, mammalian, insects and transgenic animal and plants Scientific and technical approaches to accelerating the development of robust upstream and downstream processes Identify the limitations in developing processes for antibody production