Viral Applications of Green Fluorescent Protein
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Contains protocols useful for both basic researchers and for those interested in applied viral gene therapyIncludes viral methods for terminally differentiated cells and for undifferentiated cells, including human stem cellsProvides example applications for many different viral vectors including HIV, herpes virus, pox virus, cytomegalovirus, adenovirus and adeno-associated viruses, baculovirus, SARS, and many othersExamines state-of-the-art imaging techniques including in vivo two-color fluorescence video micrsocopy and the combination of fluorescence imaging with PET, SPECT or MRIDescribes viral applications applicable for Medicine, Neuroscience, Immunology, Agriculture and Laboratory Safety